By Minoru Ueda (Ed.)
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Extra resources for Applied Tissue Engineering
VEGF) and bone regeneration emphasizes the important role of vasculature not only for survival but also for the proper formation of tissue-engineered bone. In 1997, Asahara et al. characterized endothelial progenitor cells (EPCs) in human peripheral blood using magnetic beads selection . Since EPCs can give rise to endothelial cells (ECs) and are known to facilitate the neovascularization of an implanted site, EPCs may be used to facilitate collateral vessel growth into ischemic tissues through delivery of antiangiogenic or proangiogenic agents.
Distraction of the transport bony segment has been attempted for closing alveolar defects. The defects are actually only reduced and not eliminated, and the teeth in the transport segment also were moved unintentionally according to the distraction. Some alteration in teeth positions may be beneficial, but others compromise crown morphology or require its recontouring. The bone transport in repair of the alveolar cleft therefore remains controversial (Fig. 23). Injectable tissue-engineered bone thus has a promising future.
F) Injection of tissue engineered bone (From Ueda et al. 2008). Preparation of injectable tissue-engineered bone PRP was stirred and stored at 22°C in a conventional shaker until used. Powdered human thrombin (500 units) was dissolved in 10% calcium chloride in a separate sterile cup. 5 ml sterile syringe. 5 ml syringe. The two syringes were connected with a T connector, and the plungers of the syringe were 32 Applied Tissue Engineering pushed and pulled alternatively, allowing air bubbles to flow back and forth between the two syringes.
Applied Tissue Engineering by Minoru Ueda (Ed.)