By Nitar Nwe
This concise monograph sequence specializes in the implementation of varied engineering ideas within the perception, layout, improvement, research and operation of biomedical, biotechnological and nanotechnology platforms and functions. Authors are inspired to publish their paintings within the following center issues, yet authors should still touch the commissioning editor prior to filing a suggestion.
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Extra info for Biopolymer Based Micro- and Nano-Materials
After homogeneous solution was obtained, 8 g of alginate solution was transferred to a cultivation dish (dimensions = diameter 35 mm) and incubated at room temperature for 7 h to remove air bubbles and then frozen at –20 oC. After that samples were freeze-dried. , 2013, Marine Biomaterials: Characterization, Isolation and Application, (pp. 45–60). CRC press, Taylor & Francis Group, New York. ) . Preparation of Macro-, Micro- and Nano-Materials 39 rapidly in a neutral aqueous medium. 6% (w/v) aqueous calcium chloride solutions dissolved in water for overnight and washed several times with distilled water to remove excess calcium chloride in and on the scaffolds.
For the wet sample, the materials was freeze-dried after by soaking in water. The dried sample was sandwiched between two pieces of sandpaper on either side of material with the help of Scotch permanent adhesive glue. 5 mm of the material strip was glued on either side to the end of the paper pieces. The tensile properties of materials were examined with an Orientec StA-1150 ( Japan) at room temperature. Sand papers were attached to both sides of the grips. The end of each grip was the beginning of the attached sample in order to prevent the deformation of samples during mounting.
38 Biopolymers Based on Micro- and Nano-Materials 4-D Preparation of micro- and nano-sized pores in scaffolds using biopolymers Preparation of chitosan scaffolds Chitosan scaffolds have been prepared by several methods. The basic principle of all methods is the same. The methods for the preparation of chitosan scaffold are (1) freeze-drying (2) freeze-gelation and (3) 3-axis robotic arm dispensing system method . Preparation of chitosan scaffold using freeze-drying method is shown in Figure 4-10.
Biopolymer Based Micro- and Nano-Materials by Nitar Nwe