By Kim R. Rogers, Ashok Mulchandani, Weichang Zhou
content material: Biomolecular sensing for bioprocess and environmental tracking purposes / Kim R. Rogers and Ashok Mulchandani --
Use of an acoustic wave machine as a liquid chromatography detector / may perhaps Tom-Moy, Thomas P. Doherty, Richard L. Baer, and Darlene Spira-Solomon --
Immunosensors for detection of chemical combinations : antibody affinities, selectivities, and cloning / Mohyee Eldefrawi, Amira Eldefrawi, Jeremy Wright, Peter Emanuel, James Valdes, and Kim R. Rogers : variation of a fiber-optic biosensor to be used in environmental tracking / Mark D. Pease, Lisa Shriver-Lake, and Frances S. Ligler --
a brand new technique for the detection and size of polyaromatic cancer agents and comparable compounds by way of DNA intercalation / John J. Horvath, Manana Gueguetchkeri, Adarsh Gupta, Devi Penumatchu, and Howard H. Weetall --
Chemically changed electrode for hydrogen peroxide dimension via relief at low strength / Ashok Mulchandani and Lisa C. Barrows --
Enzyme sensors for subnanomolar concentrations / Frieder W. Scheller, Alexander Makower, Andrey L. Ghindilis, Frank F. Bier, Eva Förster, Ulla Wollenberger, Christian Bauer, Burkhard Micheel, Dorothea Pfeiffer, Jan Szeponik, Norbert Michael, and H. Kaden --
Electroenzymatic sensing of fructose utilizing fructose dehydrogenase immobilized in a self-assembled monolayer on gold / K.T. Kinnear and H.G. Monbouquette --
contemporary advances in bioprocess tracking and regulate / Weichang Zhou and Ashok Mulchandani --
Optical size of bioprocess and scientific analytes utilizing lifetime-based section fluorimetry / Shabbir B. Bambot, Joseph R. Lakowicz, Jeffrey Sipior, Gary Carter, and Govind Rao --
Biosensor for online tracking of penicillin in the course of its creation through fermentation / Canh Tran-Minh and Helmut Meier --
Selective size of glutamine and asparagine in aqueous media through near-infrared spectroscopy / Xiangji Zhou, Hoeil Chung, Mark A. Arnold, Martin Rhiel, and David W. Murhammer --
knowledgeable approach for the supervision of a multichannel circulate injection research method / B. Hitzmann, R. Gomersall, J. Brandt, and A. van Putten --
Hybrid approach modeling for complicated method kingdom estimation, prediction, and keep watch over exemplified in a production-scale mammalian cellphone tradition / M. Dors, R. Simutis, and A. Lübbert --
Optimization of an Escherichia coli fed-batch fermentation utilizing a turbidity dimension procedure / Yinliang Chen, Alahari Arunakumari, Glen Hart, and Julia Cino --
The automation of 2 flow-injection immunoassays utilizing a versatile software program approach / B. Hitzmann, B. Schulze, M. Reinecke, and T. Scheper.
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Additional info for Biosensor and Chemical Sensor Technology. Process Monitoring and Control
4, for 30 minutes. ; ACS Symposium Series; American Chemical Society: Washington, DC, 1996. ch004 Wash H 1 0 1 1 1 100 Wash J 200 1 1 1 300 1 1 400 1 500 1 T i m e (Sec) Figure 3. Response of fluorimeter for the course of a single assay. Cy3-IgG-l and Cy3-IgG-2 are the first and second application of the antibody-dye conjugate, Cy3-IgG, respectfully. 5-, —•— PBS — ο — Ground Water τ 4 • 1 5 • 1 6 · 1 7 « r 8 Cell Concentration in log(cells/ml) Figure 4. Response curves for the G4 sandwich immunoassay in buffer and ground water, for single fibers.
4. 5. 6. 7. 8. 9. 10. 11. 12. 13. 14. 15. 16. 17. 18. 19. 20. 21. 22. 23. 24. 25. 26. 27. ; Hogrefe, H . ; Sorge, J. Strategies in Molecular Biology 1993, 5, 2-5. B. J. Agri. , 1993, 41, 843-848. ; Benkovic, S. Proc. Natl. Acad. Sci. 1991, 88, 7978-7982. H. Biochem. Pharmacol. 1989, 182, 353-359. E. Analyt. ; Hoffman T. 1983, 92, 168-174. ; Yang, M . ; O'Connell, M . ; Henner, D. Bio/Technology 1991, 9, 1373-1377. B. Appl. Opt. 1986, 26, 2181-2187. ; Schacher, A. J. Chromat. 1987, 411, 177-184.
Detection of whole cells is an ideal application of the sandwich assay since whole cells have multiple identical, non-interfering epitopes. This format was used for the detection of G4. Competitive Binding Assay. In the competitive assay, the analyte and a fluorophore-labeled analog compete for a limited number of available binding sites. The competitive assay is often employed with small molecules lacking two independent epitopes. Thus, the competitive binding assay is ideal for the detection of a small molecule like TNT.
Biosensor and Chemical Sensor Technology. Process Monitoring and Control by Kim R. Rogers, Ashok Mulchandani, Weichang Zhou