By Victor Ginsburg (Eds.)
The seriously acclaimed laboratory commonplace, Methods in Enzymology, is among the so much hugely revered guides within the box of biochemistry. considering 1955, each one quantity has been eagerly awaited, usually consulted, and praised via researchers and reviewers alike. The sequence comprises a lot fabric nonetheless correct at the present time - actually a vital book for researchers in all fields of lifestyles sciences
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Extra resources for Complex Carbohydrates Part F
Acknowledgments The author gratefully acknowledges the contributions of collaborators and colleagues, especially Dr. Bernard Kaufman, to the work presented here. These studies were supported by National Institutes of Health Grant HL33152. 47 K. R. Bhaskar and L. Reid, J. BioL Chem. 256, 7583 (1981). 48 H. Woodward, B. Horsey, V. P. Bhavanandan, and E. A. Davidson, Biochemistry 21,694 (1982). 49 N. Houdret, J. Perini, C. Galabert, A. Scharfman, P. Humbert, G. Lamblin, and P. Roussel, Biochim. Biophys.
Biol. Chem. 261, 12779 (1986). 7 S. R. Carlsson, H. Sasaki, and M. Fukuda, J. Biol. Chem. 261, 12787 (1986). s M. Fukuda, S. R. Carlsson, J. C. Klock, and A. Dell, J. Biol. Chem. 261, 12796 (1986). 9 F. Piller, V. Piller, R. I. Fox, and M. Fukuda, J. Biol. Chem. 263, 15146 (1988).  O - L I N K E D SACCHARIDES FROM CELL SURFACE GLYCOPROTEINS 19 Isolation and Structural Characterization of O-Linked Oligosaccharides Preparation of O-Linked Oligosaccharidesfrom Whole Cells or Membranes. When preparation of O-linked oligosaccharides begins with whole cells or membranes, the sample is first delipidated with organic solvents.
This is because O-linked oligosaccharides are attached to a polypeptide as a cluster so that the region enriched in O-linked saccharides is resistant to protease) 1 In fact, this fraction of high molecular weight can be converted to O-linked oligosaccharides by alkaline borohydride treatment, as follows. Fractions containing larger glycopeptides from the above Sephadex G-50 gel filtration are pooled and desalted. If this fraction still contains a significant amount of peptides, the glycopeptide fractions are further purified by DE-52 column chromatography as described above.
Complex Carbohydrates Part F by Victor Ginsburg (Eds.)