By Thomas Wartmann, Gotthard Kunze (auth.), Prof. Dr. Klaus Wolf, Prof. Dr. Karin Breunig, Prof. Dr. Gerold Barth (eds.)
Most details on yeasts derives from experiments with the normal yeasts Saccaromyces cerevisiae and Schizossaccharomyces pombe, the entire nuclear and mitochondrial genome of which has additionally been sequenced. For all different non-conventional yeasts, investigations are in growth and the fast improvement of molecular suggestions has allowed an perception additionally right into a number of non-conventional yeasts.
In this bench handbook, over 70 useful protocols utilizing 15 various non-conventional yeast species and likewise numerous protocols of common use are defined intimately. All of those experiments at the genetics, biochemistry and biotechnology of yeasts were contributed via popular laboratories and feature been reproduced repeatedly. The trustworthy protocols are therefore supreme additionally for undergraduate and graduate useful courses.
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So much info on yeasts derives from experiments with the normal yeasts Saccaromyces cerevisiae and Schizossaccharomyces pombe, the whole nuclear and mitochondrial genome of which has additionally been sequenced. For all different non-conventional yeasts, investigations are in development and the speedy improvement of molecular thoughts has allowed an perception additionally right into a number of non-conventional yeasts.
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Extra info for Non-Conventional Yeasts in Genetics, Biochemistry and Biotechnology: Practical Protocols
Niger), Ca-GCN4 (C. albicans), and GCN4 (S. cerevisiae). Designed forward and reverse PCR primers to amplify a part of C-GCN4 5 A GCN4 Gene Analogue and Its Involvement in Cycloheximide Resistance C-ADE1 or C-HIS5 Marker gene C-GCN4 Afl II Open Reading Frame Bst 1107I C-ADE1 or C-HIS5 Marker gene C-GCN4 Open Reading Frame Fig. 2. Gene disruption of C-GCN4. C-ADE1 or C-HIS5 was used as a marker gene In C. maltosa, the expression of L41-Qs is required for the induction of resistance to CYH (Mutoh et al.
J Biol Chem 274:9442–9448 Hanson AM (1967) Microbial production of pigments and vitamins. In: Peppler HJ (ed) Microbial technology. Reinhold, New York, pp 222–250 Kurth R, Philippsen P, Steiner S,Wright M (1992) New promotor region. Patent cooperation treaty no WO 92/00379 Monschau N, Stahmann KP, Sahm H, McNeil JB, Bognar AL (1997) Identification of Saccharomyces cerevisiae GLY1 as a threonine aldolase: a key enzyme in glycine biosynthesis. FEMS Microbiol Lett 150:55–60 Monschau N, Sahm H, Stahmann KP (1998) Threonine aldolase overexpression plus threonine supplementation enhanced riboflavin production in Ashbya gossypii.
Cpc1p (Neurospora (Neurospora crassa) crassa) CpcAp (Aspergillus (Aspergillus niger) niger) (Candida albicans) Ca-GCN4p (Candida albicans ) GCN4p (Saccharomyces cerevisiae ) (Saccharomyces cerevisiae) PLPPIIVEDPSDVVAMKRARNTLAARKSRERKAQRLEELEAKIEEL PLPPIKF-DSADPAAMKRARNTEAARKSRARKLERQGEMERRIEEL PLQPIVVDDIKDAAALKRAKNTEAARRSRARKMERMSQLEDKVENL PLSPIVPESS-DPAALKRARNTEAARRSRARKLQRMKQLEDKVEEL ** ** . *:***:** ***:** ** :* PLQPIV 5'-CCWTTRCARCCWATHGT-3' ::* ::*:* QLEDKVE 5'-TTCNACYTTRTCTTCHARTTG-3' Forward primer Reverse primer R : A or G W : A or T H : A or C or T Y : C or T N : A or C or G or T Fig.
Non-Conventional Yeasts in Genetics, Biochemistry and Biotechnology: Practical Protocols by Thomas Wartmann, Gotthard Kunze (auth.), Prof. Dr. Klaus Wolf, Prof. Dr. Karin Breunig, Prof. Dr. Gerold Barth (eds.)